Ion Channels: Channel Biochemistry, Reconstitution, and Function

<p>Preface</p> <p>Daniel L. Minor Jr. and Henry M. Colecraft</p> <p>Structure/function relationships</p> <p>1. Correlating ion channel structure and function</p> <p>Crina Nimigean</p> <p>2. TRPV3 Expression and Purification for Structure Determination by Cryo-EM</p> <p>Alexander Sobolevsky</p> <p>3. Methods to study phosphoinositide regulation of ion channels</p> <p>Tibor Rohacs</p> <p>4. Recombinant Expression and Purification of Pentameric Ligand-Gated Ion Channels for Cryo-EM Structural Studies </p> <p>Sudha Chakarpani</p> <p>5. Detection of ligand binding to purified HCN channels using fluorescence-based size exclusion chromatography</p> <p>Anna Moroni</p> <p>Structural and chemical methods to assess channel dynamics</p> <p>6. Structural Dynamics of Channels and Transporters by High-Speed Atomic Force Microscopy </p> <p>Simon Scheuring</p> <p>7. Crosslinking for gating motions in GluRs</p> <p>Andrew Plested</p> <p>8. Single molecule FRET methodology for investigating glutamate receptors</p> <p>Vasanthi Jayaraman</p> <p>9. Interrogating permeation and gating of Orai channels using chemical modification of cysteine residues</p> <p>Murali Prakriya</p> <p>Channel reconstitution and studies in bilayers</p> <p>10. A quantitative flux assay for the study of Cl- channels and transporters</p> <p>Alessio Accardi</p> <p>11. Ion channels reconstitution in lipid bilayers</p> <p>Eleonora Zakharian</p> <p>12. Combining in vitro translation with nanodisc technology and functional reconstitution of channels in planar lipid bilayers</p> <p>Gehard Thiel</p> <p>Methods to assess oligomerization</p> <p>13. The application of Poisson distribution statistics in ion channel reconstitution to determine oligomeric architecture</p> <p>Randy Stockbridge</p>