Enzyme Engineering and Evolution: Specific Enzyme Applications
Specificaties
Gebonden, blz.
|
Engels
Elsevier Science |
e druk, 2020
ISBN13: 9780128244319
Rubricering
Onderdeel van serie
Methods in Enzymology
Verwachte levertijd ongeveer 9 werkdagen
Samenvatting
Methods in Enzymology, Volume 644, the latest release in this ongoing serial, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Site-directed recombination (SDR) in vivo: a fast and reliable tool to unveil beneficial epistasis, Creation and application of amine oxidase with expanded substrate specificities from porcine kidney D-amino acid oxidase, Methods to assess correlation networks for engineering transketolase, Exploration of Enzyme Diversity by Integrating Bioinformatics with Microfluidics, Engineering lytic polysaccharide monooxygenases (LPMOs), Emulsion-based directed evolution of enzymes in yeast, and much more.
Specificaties
ISBN13:9780128244319
Taal:Engels
Bindwijze:Gebonden
Uitgever:Elsevier Science
Serie:Methods in Enzymology
Inhoudsopgave
<p>1. Engineering lytic polysaccharide monooxygenases (LPMOs)</p> <p>Zarah Forsberg, Anton A. Stepnov, Guro Kruge Nærdal, Geir Klinkenberg and Vincent G. H. Eijsink</p> <p>2. Strategies for the expression and characterization of artificial myoglobin-based carbene transferases</p> <p>Daniela M. Carminati, Eric J. Moore and Rudi Fasan</p> <p>3. Methods for the detection and analysis of dioxygenase catalyzed dihydroxylation in mutant derived libraries</p> <p>Julian L. Wissner, Wendy Escobedo-Hinojosa, Peter M. Heinemann, Andreas Hunold and Bernhard Hauer</p> <p>4. Enhancing promiscuous chemistries of a Schiff-base forming enzyme by divergent evolution</p> <p>Duncan S. Macdonald and Donald Hilvert</p> <p>5. Glutamine-walking: Creating reactive substrates for transglutaminase-mediated protein labeling</p> <p>Lukas Deweid, Adem H. Parisien, Kiana Lafontaine, Léa N.C. Rochet, Harald Kolmar and Joelle N. Pelletier</p> <p>6. Engineering aldolases for asymmetric synthesis</p> <p>Mikael Widersten</p> <p>7. Ultra-high throughput screening for novel protease specificities</p> <p>Eirini Rousounelou, Steven Schmitt, Luzius Pestalozzi, Martin Held, Tania M. Roberts and Sven Panke</p> <p>8. Directed evolution of transcriptional switches using dual-selector systems</p> <p>Yuki Kimura and Daisuke Umeno</p> <p>9. Methods for competitive enrichment and evaluation of superior DNA ligases</p> <p>Janine K. Sharma, Luke J. Stevenson, Katherine J. Robins, Adele K. Williamson, Wayne M. Patrick and David F. Ackerley</p> <p>10. Directed evolution of custom polymerases using droplet microfluidics</p> <p>Derek Vallejo, Ali Nikoomanzar and John C. Chaput</p> <p>11. Optically guided mass spectrometry to screen microbial colonies for directed enzyme evolution</p> <p>Pu Xue, Tong Si and Huimin Zhao</p> <p>12. Split enzymes: Design principles and strategy</p> <p>Shion A. Lim and James A. Wells</p>