Liquid-Liquid Phase Coexistence and Membraneless Organelles

Specificaties
Gebonden, blz. | Engels
Elsevier Science | e druk, 2021
ISBN13: 9780128211595
Rubricering
Elsevier Science e druk, 2021 9780128211595
Onderdeel van serie Methods in Enzymology
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Samenvatting

Methods in Enzymology, Volume 646, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Methods for Studying RNA condensation/granules in vitro, RNA Dynamics in Intracellular Condensates, Methods for Viscoelastic Characterization of Liquid and Gel Condensates, Incorporating Proteins into Complex Coacervates, Methods for Study of Liquid-Liquid Phase Coexistence in Proximity to Lipid Membranes, Preparation of and Solute Partitioning in Multiphase Coacervates, Reversible photocontrol of DNA coacervation, Enzymatic Control over Coacervation, and much more.

Specificaties

ISBN13:9780128211595
Taal:Engels
Bindwijze:Gebonden

Inhoudsopgave

<p>1. Using a sequence-specific coarse-grained model for studying protein liquid–liquid phase separation</p> <p>Roshan M. Regy, Wenwei Zheng and Jeetain Mittal</p> <p>2. Reconstituting bacterial cell division assemblies in crowded, phase-separated media</p> <p>Begoña Monterroso, Miguel Ángel Robles-Ramos, Silvia Zorrilla and Germán Rivas</p> <p>3. Terpolymer-stabilized complex coacervates: A robust and versatile synthetic cell platform</p> <p>Alexander F. Mason, Wiggert J. Altenburg, Shidong Song, Marleen van Stevendaal and Jan C.M. van Hest</p> <p>4. Cell-free reconstitution of multi-condensate assemblies</p> <p>Andrea Putnam and Geraldine Seydoux</p> <p>5. Practical considerations for generation of multi-compartment complex coacervates</p> <p>Gregory A. Mountain and Christine D. Keating</p> <p>6. Methods for characterizing the material properties of biomolecular condensates</p> <p>Ibraheem Alshareedah, Taranpreet Kaur and Priya R. Banerjee</p> <p>7. Small-angle X-ray scattering experiments of monodisperse intrinsically disordered protein samples close to the solubility limit</p> <p>Erik W. Martin, Jesse B. Hopkins and Tanja Mittag</p> <p>8. SAXS methods for investigating macromolecular and self-assembled polyelectrolyte complexes</p> <p>Amanda B. Marciel, Samanvaya Srivastava, Jeffrey M. Ting and Matthew V. Tirrell</p> <p>9. Ultra-small angle neutron scattering to study droplet formation in polyelectrolyte complex coacervates</p> <p>Vivek M. Prabhu, Samim Ali, Markus Bleuel, Yimin Mao and Yuanchi Ma</p> <p>10. Incorporation of proteins into complex coacervates</p> <p>Whitney C. Blocher McTigue and Sarah L. Perry</p> <p>11. Measuring the activity and structure of functional RNAs inside compartments formed by liquid-liquid phase separation</p> <p>Raghav R. Poudyal, McCauley O. Meyer and Philip C. Bevilacqua</p> <p>12. Reversible photocontrol of DNA coacervation</p> <p>Suzanne Lafon and Nicolas Martin</p> <p>13. Enzymatic control over coacervation</p> <p>Karina K. Nakashima, Alain A.M. André and Evan Spruijt</p>
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        Liquid-Liquid Phase Coexistence and Membraneless Organelles