Chemical and Synthetic Biology Approaches to Understand Cellular Functions - Part C

<p>1. Competitive binding assay for biotin and biotin derivatives, based on avidin and biotin-4-fluorescein<br>Elke Oberbichler, Maria Wiesauer, Eva Schlögl, Jessica Stangl, Felix Faschinger, Günther Knör, Hermann J. Gruber and Vesa P. Hytönen<br>2. (Strept)avidin as a template for ligands other than biotin: An overview<br>Vesa P. Hytönen<br>3. Engineering siderophores<br>Sina Rütschlin and Thomas Böttcher<br>4. Competitive profiling for enzyme inhibitors using chemical probes<br>Michaela Prothiwa and Thomas Böttcher<br>5. The NMR tube bioreactor<br>Alexandra V. Chatzikonstantinou, Antonis Tsiailanis, Ioannis P. Gerothanassis, Haralambos Stamatis, Enrico Ravera, Marco Fragai, Claudio Luchinat, Giacomo Parigi and Andreas G. Tzakos<br>6. A chemically-controlled system for activating RAS GTPases<br>Emily M. Dieter and Dustin J. Maly<br>7. Temporal and rheostatic control of genome editing with a chemically-inducible Cas9<br>Cindy T. Wei, Dustin J. Maly and Douglas M. Fowler<br>8. Synthetic receptors to understand and control cellular functions<br>Hung-Ju Chang and Jerome Bonnet<br>9. A suite of bioassays to evaluate CREB inhibitors<br>Bingbing X. Li and Xiangshu Xiao<br>10. Identification of lamins as the molecular targets of LBL1 using a clickable photoaffinity probe<br>Xiangshu Xiao and Bingbing X. Li<br>11. REX technologies for profiling and decoding the electrophile signaling axes mediated by Rosetta Stone proteins<br>Marcus J. C. Long, Daniel A. Urul and Yimon Aye<br>12. Building artificial genetic circuits to understand protein function<br>Louis H. Scott, James C. Mathews, Aleksandra Filipovska and Oliver Rackham<br>13. Methods for studying human sirtuins with activity-based chemical probes<br>Song Zheng, Jessica Wohlfahrt, Ian Cohen and Yana Cen<br>14. Site-directed labeling of β-arrestin with monobromobimane for measuring their interaction with G protein-coupled receptors<br>Ashish Srivastava, Mithu Baidya, Hemlata Dwivedi-Agnihotri and Arun K. Shukla<br>15. Reversible biotinylation of purified proteins for measuring protein-protein interactions<br>Hemlata Dwivedi-Agnihotri, Ashish Srivastava and Arun K. Shukla</p>