Tumor Immunology and Immunotherapy – Cellular Methods Part A

<p>1. Cytokine profiling of tumor-infiltrating T lymphocytes by flow cytometry<br>Jeremy B. Foote, Sujith Sarvesh and Leisha A. Emens<br>2. High-throughput identification of human antigen-specific CD8<SUP>+</SUP> and CD4<SUP>+</SUP> T cells using soluble pMHC multimers<br>Morgane Magnin, Philippe Guillaume, George Coukos, Alexandre Harari and Julien Schmidt<br>3. In vitro assays for effector T cell functions and activity of immunomodulatory antibodies<br>Roberta Zappasodi, Sadna Budhu, Mohsen Abu-Akeel and Taha Merghoub<br>4. A cytofluorimetric assay to evaluate T cell polyfunctionality<br>Belinda Palermo, Mariangela Panetta, Giulia Campo and Paola Nisticò<br>5. Assessment of memory formation by metabolically engineered antigen-specific CD8 T cells<br>Mathias Wenes, Pedro Romero and Lianjun Zhang<br>6. Functional characterization of tumor antigen-specific T-cells isolated from the tumor microenvironment of sleeping beauty induced murine glioma models<br>Mahmoud S. Alghamri, Felipe J. Núñez, Neha Kamran, Stephen Carney, David Altshuler, Pedro R. Lowenstein and Maria G. Castro<br>7. Methods to edit T cells for cancer immunotherapy<br>Francesca Lucibello, Silvia Menegatti and Laurie Menger<br>8. Generating stem-like memory T cells with antioxidants for adoptive cell transfer immunotherapy of cancer<br>Karolina Pilipow, Eloise Scamardella and Enrico Lugli<br>9. Reverse immunology: From peptide sequence to tumor-killing human T-cell clones<br>Christophe Vanhaver, Monica Gordon-Alonso, Alexandre Bayard, Maria Teresa Catanese, Didier Colau, Pierre van der Bruggen and Annika M. Bruger<br>10. Generation of TCR-engineered reference cell samples to control T-cell assay performance<br>Nicole Bidmon, Cécile Gouttefangeas and Sjoerd H. van der Burg<br>11. In vitro expansion of Vγ9Vδ2 T cells for immunotherapy<br>Christian Peters, Léonce Kouakanou, Hans-Heinrich Oberg, Daniela Wesch and Dieter Kabelitz<br>12. CFSE dilution to study human T and NK cell proliferation in vitro<br>Iñigo Terrén, Ane Orrantia, Joana Vitallé, Olatz Zenarruzabeitia and Francisco Borrego<br>13. Rapid isolation and enrichment of mouse NK cells for experimental purposes<br>Maite Alvarez, Maria C. Ochoa, Luna Minute, Ignacio Melero and Pedro Berraondo<br>14. Assessment of NK cell-mediated cytotoxicity by flow cytometry after rapid, high-yield isolation from peripheral blood<br>Peter Holicek, Iva Truxova, Lenka Kasikova, Sarka Vosahlikova, Cyril Salek, Jana Rakova, Monika Holubova, Daniel Lysak, Isabelle Cremer, Radek Spisek and Jitka Fucikova<br>15. Two-dimensional dynamic evaluation of natural killer cell-mediated lysis of adherent target cells<br>Alexandra Frazao, Louise Rethacker and Anne Caignard<br>16. Rapid isolation of mouse ILCs from murine intestinal tissues<br>Kyle Burrows, Pailin Chiaranunt, Louis Ngai and Arthur Mortha<br>17. Detecting and analyzing murine innate lymphoid cells<br>Alejandra Gomez-Cadena, Pedro Romero, Sara Trabanelli and Camilla Jandus<br>18. A cytofluorimetric assay to evaluate intracellular cytokine production by NK cells<br>Christian Sordo-Bahamonde, Seila Lorenzo-Herrero, Segundo González and Alejandro López-Soto<br>19. Applications of microfluidic devices in advancing NK-cell migration studies<br>Xiaoou Ren, Abdulaziz Alamri, Jolly Hipolito, Francis Lin and Sam K.P. Kung<br>20. Complementary approaches to study NKT cells in cancer<br>Jay A. Berzofsky, Purevdorj B. Olkhanud and Masaki Terabe<br>21. Assessment of IFN-γ and granzyme-B production by in "sitro" technology<br>Claudia Galassi, Gwenola Manic, Martina Musella, Antonella Sistigu and Ilio Vitale<br>22. Assessment of IFNγ responsiveness in patient-derived xenografts<br>Jordan J. Cardenas, Camila Robles-Oteiza and Katerina Politi<br>23. Real-time cell analysis (RTCA) to measure killer cell activity against adherent tumor cells in vitro<br>Hans-Heinrich Oberg, Christian Peters, Dieter Kabelitz and Daniela Wesch<br>24. Analysis of cancer cell-intrinsic immune regulation in response to CD8+ T cell attack<br>Natalie J. Neubert, Laure Tillé, Christophe Martignier, Silvia A. Fuertes Marraco and Daniel E. Speiser<br>25. A flow cytometry-based method to screen for modulators of tumor-specific T cell cytotoxicity<br>Javier Santos, Jesús Ogando, Rosa Ana Lacalle and Santos Mañes<br>26. Evaluation of NK cell cytotoxic activity against malignant cells by the calcein assay<br>Seila Lorenzo-Herrero, Christian Sordo-Bahamonde, Segundo González and Alejandro López-Soto<br>27. 51Cr-release to monitor NK cell cytotoxicity<br>Leslie Elsner and Ralf Dressel</p>