Ubiquitin-dependent Protein Degradation
Specificaties
Gebonden, blz.
|
Engels
Elsevier Science |
e druk, 2019
ISBN13: 9780128186671
Rubricering
Onderdeel van serie
Methods in Enzymology
Levertijd ongeveer 8 werkdagen
Samenvatting
Ubiquitination and Protein Stability - Part B, Volume 619, the latest release in the Methods in Enzymology series, highlights new advances in the field, with this updated volume presenting interesting chapters written by an international board of authors. Topics of note include chapters on Assays of SUMO protease function in mammalian cells, In vitro analysis of proteasome-associated USP14 activity for substrate degradation and deubiquitylation, Methods to study proteasome regulatory particle assembly, Native mass spectrometry approaches to study the proteasome, Single-molecule methods to study the ubiquitin-proteasome system, Assays for the function of ubiquitin in the mammalian endocytic pathway, and much more.
Specificaties
ISBN13:9780128186671
Taal:Engels
Bindwijze:Gebonden
Uitgever:Elsevier Science
Serie:Methods in Enzymology
Inhoudsopgave
<p>1. Assays for protein retrotranslocation in ERAD<br>Sonya Neal, Sascha H. Duttke and Randolph Y. Hampton<br>2. Methods for measuring misfolded protein clearance in the budding yeast Saccharomyces cerevisiae<br>Rahul S. Samant and Judith Frydman<br>3. Methodologies to monitor protein turnover at the inner nuclear membrane <br>Pei-Ling Tsai, Chenguang Zhao and Christian Schlieker<br>4. Assays for dissecting the in vitro enzymatic activity of yeast Ubc7 <br>Bayan Mashahreh, Yuval Reiss, Reuven Wiener and Tommer Ravid<br>5. Methods for genetic analysis of mammalian ER-associated degradation<br>Dara E. Leto and Ron R. Kopito<br>6. A dual system to manipulate protein levels for DNA replication- and cell cycle-related studies<br>Néstor García-Rodríguez and Helle D. Ulrich<br>7. Engineered disulfide crosslinking to measure conformational changes in the 26S proteasome<br>Randi G. Reed and Robert J. Tomko Jr.<br>8. Assays for ubiquitin-like protein ligation and proteasome function in archaea<br>Xian Fu, Zachary Adams and Julie Maupin-Furlow<br>9. Structural mass spectrometry approaches to study the 20S proteasome<br>Gili Ben-Nissan, Shay Vimer, Mark Tarnavsky and Michal Sharon<br>10. Single-molecule methods for measuring ubiquitination and protein stability<br>Jason Hon and Ying Lu<br>11. In vitro analysis of proteasome-associated USP14 activity for substrate degradation and deubiquitylation<br>Srinivasan Muniyappan and Byung-Hoon Lee<br>12. Methods for studying the regulation of membrane traffic by ubiquitin and the ESCRT pathway<br>Evan L. Guiney, Lu Zhu, Richa Sardana, Scott D. Emr and Matthew G. Baile<br>13. Detection of ubiquitinated targets in mammalian and Drosophila models<br>Elena Maspero, Valentina Fajner, Janine Weber and Simona Polo<br>14. Western blot analysis of the autophagosomal membrane protein LGG-1/LC3 in Caenorhabditis elegans<br>Alexander Springhorn and Thorsten Hoppe<br>15. Monitoring stress-induced autophagic engulfment and degradation of the 26S proteasome in mammalian cells<br>Victoria Cohen-Kaplan, Ido Livneh, Yong Tae Kwon and Aaron Ciechanover</p>