Ubiquitin and Ubiquitin-like Protein Modifiers
Specificaties
Gebonden, blz.
|
Engels
Elsevier Science |
e druk, 2019
ISBN13: 9780128163597
Rubricering
Onderdeel van serie
Methods in Enzymology
Levertijd ongeveer 8 werkdagen
Samenvatting
Ubiquitination and Protein Stability - Part A Volume 618, the latest release in the Methods in Enzymology series, highlights new advances in the field, with this updated volume presenting interesting chapter written by an international board of authors. Topics of note in this new release include the Preparation of ubiquitinated nucleosomes with native and non-hydrolyzable linkages, Methods to measure ubiquitin chain length and linkage, Genetic approaches to study the yeast ubiquitin system, Enzymatic preparation of monoubiquitinated proteins, Methods to distinguish the function of ubiquitin in autophagy and the proteasome pathway, the Purification and characterization of enzyme activity of USPs, and much more.
Specificaties
ISBN13:9780128163597
Taal:Engels
Bindwijze:Gebonden
Uitgever:Elsevier Science
Serie:Methods in Enzymology
Inhoudsopgave
<p>1. Semisynthesis of ubiquitinated histone H2B with a native or nonhydrolyzable linkage<br>Michael Morgan, Muhammad Jbara, Ashraf Brik and Cynthia Wolberger<br>2. Dual-color pulse-chase ubiquitination assays to simultaneously monitor substrate priming and extension<br>Daniel C. Scott and Brenda A. Schulman<br>3. A genetic approach to study polyubiquitination in Saccharomyces cerevisiae<br>Fernando Meza-Gutierrez, Deniz Simsek and David P. Toczyski<br>4. Enzymatic preparation of monoubiquitinated FANCD2 and FANCI proteins<br>Viduth K. Chaugule, Connor Arkinson, Rachel Toth and Helen Walden<br>5. Methods to measure ubiquitin chain length and linkage<br>Fumiaki Ohtake, Hikaru Tsuchiya, Keiji Tanaka and Yasushi Saeki<br>6. Detection of ubiquitination activity and identification of ubiquitinated substrates using TR-TUBE<br>Yukiko Yoshida, Yasushi Saeki, Hikaru Tsuchiya and Keiji Tanaka<br>7. Methods to study phosphoribosylated ubiquitin ligation and removal<br>Jiazhang Qiu and Zhao-Qing Luo<br>8. Biochemical characterization of SUMO-conjugating enzymes by in vitro sumoylation assays<br>Nathalie Eisenhardt, Dragana Ilic, Easa Nagamalleswari and Andrea Pichler<br>9. Methods to study SUMO dynamics in yeast<br>Stefan Pabst, Lennard-Maximilian Döring, Natasha Petreska and R. Jürgen Dohmen<br>10. Approaches for investigating the extracellular signaling function of ISG15<br>Caleb D. Swaim, Larissa A. Canadeo and Jon M. Huibregtse<br>11. Analysis of modification and proteolytic targeting by the ubiquitin-like modifier FAT10<br>Annette Aichem, Annika N. Boehm, Nicola Catone, Gunter Schmidtke and Marcus Groettrup<br>12. Methods to analyze STUbL activity<br>Emma Branigan, Anna Plechanovová and Ronald T. Hay<br>13. Quantitative analysis of USP activity in vitro<br>Shreya Dharadhar, Robbert Q. Kim, Michael Uckelmann and Titia K. Sixma<br>14. Evaluating enzyme activities and structures of DUBs<br>Jonathan N. Pruneda and David Komander<br>15. Purification and functional characterization of the DUB domain of SdeA<br>Kedar Puvar, Shalini Iyer, Michael J. Sheedlo and Chittaranjan Das<br>16. Profiling DUBs and Ubl-specific proteases with activity-based probes<br>Paul P. Geurink, Gerbrand J. van der Heden van Noort, Monique P.C. Mulder, Robert C.M. Knaap, Marjolein Kikkert and Huib Ovaa<br>17. Assays of SUMO protease/isopeptidase activity and function in mammalian cells and tissues<br>Kathrin Kunz, Stefan Müller and Luca Mendler</p>