Echinoderms

<p>Section 1 Procurement and culturing of established and emerging echinoderm models<br>1. Procuring animals and culturing of eggs and embryos<br>Nikki L. Adams, Andreas Heyland, Linda L. Rice and Kathy R. Foltz<br>2. Cryopreservation of sea urchin sperm and early life stages<br>Estafania Paredes, Serean L. Adams and Julien Vignier<br>3. Temnopleurus as an emerging echinoderm model<br>Shunsuke Yaguchi<br>4. Cidaroids, clypeasteroids, and spatangoids: Procurement, culture, and basic methods <br>Taku Hibino, Takuya Minokawa and Atsuko Yamazaki<br>5. The painted sea urchin, Lytechinus pictus, as a genetically-enabled developmental model<br>Katherine T. Nesbit, Travis Fleming, Grant Batzel, Amara Pouv, Hannah Rosenblatt, Douglas A. Pace, Amro Hamdoun and Deirdre C. Lyons<br>6. Culturing echinoderm larvae through metamorphosis<br>Jason Hodin, Andreas Heyland, Annie Mercier, Bruno Pernet, David L. Cohen, Jean-François Hamel, Jonathan D. Allen, Justin S. McAlister, Maria Byrne, Paula Cisternas and Sophie B. George</p> <p>Section 2 Experimental embryology approaches<br>7. Microinjection methods for sea urchin eggs and blastomeres<br>Junko Yaguchi<br>8. Microinjection of oocytes and embryos with synthetic mRNA encoding molecular probes<br>George von Dassow, Jenna Valley and Kara Robbins<br>9. Methods for transplantation of sea urchin blastomeres<br>Andrew N. George and David R. McClay<br>10. Sea urchin embryonic cilia<br>Robert L. Morris and Victor D. Vacquier<br>11. Visualizing egg and embryonic polarity<br>Lauren T. Smith and Athula H. Wikramanayake<br>12. Methods to label, isolate, and image sea urchin small micromeres, the primordial germ cells (PGCs)<br>Joseph P. Campanale, Amro Hamdoun, Gary M. Wessel, Yi-Hsien Su and Nathalie Oulhen<br>13. Culture of and experiments with sea urchin embryo primary mesenchyme cells <br>Bradley Moreno, Allessandra DiCorato, Alexander Park, Kellen Mobilia, Regina Knapp, Reiner Bleher, Charlene Wilke, Keith Alvares and Derk Joester</p> <p>Section 3 Approaches for assessing environmental influences on adults and embryos<br>14. Analysis of immune response in the sea urchin larva<br>Katherine M. Buckley, Nicholas W. Schuh, Andreas Heyland and Jonathan P. Rast<br>15. Methods for collection, handling, and analysis of sea urchin coelomocytes<br>L. Courtney Smith, Teresa S. Hawley, John H. Henson, Audrey J. Majeske, Matan Oren and Benyamin Rosental<br>16. Measurement of feeding rates, respiration, and pH regulatory processes in the light of ocean acidification research<br>Meike Stumpp, Sam Dupont and Marian Y. Hu<br>17. Methods for toxicology studies in echinoderm embryos and larvae<br>Cristina Torres-Duarte, Carol A. Vines, Elise Fairbairn and Gary N. Cherr</p> <p>Section 4 Sea urchins in the classroom<br>18. A teaching laboratory on the activation of xenobiotic transporters at fertilization of sea urchins<br>Lauren E. Shipp, Rose Z. Hill and Amro Hamdoun<br>19. Exploring the sea urchin genome with undergraduates using bioinformatic tools<br>Laura Romano, Christine Byrum, Pei Yun Lee and Robert Morris<br>20. Analyzing gene expression in sea star eggs and embryos using bioinformatics<br>Lauren Bates, Emily Wiseman and David J. Carroll</p>