Enzymes in Synthetic Biology

<p>Section I: Enzyme Discovery<br>1. Enzyme Discovery: Enzyme Selection and Pathway Design<br>P. Carbonell, Mathilde Koch, Thomas Duigou and J.L. Faulon</p> <p>Section II: Enzyme Engineering Tools and Platforms<br>2. Cell-Free Synthetic Biology for Pathway Prototyping<br>Ashty S. Karim and Michael C. Jewett<br>3. Fast and Flexible Synthesis of Combinatorial Libraries for Directed Evolution<br>Joanna C. Sadler, Lucy Green, Neil Swainston, Douglas B. Kell and <br>Andrew Currin</p> <p>Section III: Enzyme Families: Parts and Platforms for Chemical Diversity<br>4. Sesquiterpene Synthase Catalyzed Conversion of a Farnesyl Diphosphate Analogue to a Non-natural Terpenoid Ether<br>Florence Huynh David J. Miller and Rudolf K. Allemann<br>5. In Vivo Platforms for Terpenoid Overproduction and the Generation of Chemical Diversity<br>Guangkai Bian, Tian Ma and Tiangang Liu<br>6. Imine Reductases, Reductive Aminases, and Amine Oxidases for the Synthesis of Chiral Amines: Discovery, Characterization, and Synthetic Applications<br>S.C. Cosgrove, A. Brzezniak, S.P. France, J.I. Ramsden, J. Mangas-Sanchez, S.L. Montgomery, R.S. Heath and N.J. Turner<br>7. Experimental Protocols for Generating Focused Mutant Libraries and Screening for Thermostable Proteins<br>M.J.L.J. Fürst, C. Martin, N. Lončar and M.W. Fraaije<br>8. Characterization of Cytochrome P450 Enzymes and their Applications in Synthetic Biology<br>L. N. Jeffreys, H. M. Girvan, K. J. McLean and A. W. Munro</p> <p>Section IV: Genome Engineering for Enzyme Pathways<br>9. Metabolic Engineering of Saccharomyces cerevisiae Using a Trifunctional CRISPR/Cas System for Simultaneous Gene Activation, Interference, and Deletion<br>Carl Schultz, Jiazhang Lian and Huimin Zhao<br>10. YeastFab: High Throughput Genetic Parts Construction, Measurement and Pathway Engineering in Yeast<br>Eva Garcia-Ruiz, Jamie Auxillos, Tianyi Li, Junbiao Dai and Yizhi Cai</p> <p>Section V: Bioengineered Strains with Designed Enzyme Pathways<br>11. Halomonas and Pathway Engineering for Bioplastics Production<br>Jiang Xiao-Ran, Yin Jin, Chen Xiangbin and Chen Guo-Qiang<br>12. An Engineering Approach for Rewiring Microbial Metabolism<br>Sebastian Wenk, Oren Yishai, Steffen N. Lindner and Arren Bar-Even<br>13. Multifragment DNA Assembly of Biochemical Pathways via Automated Ligase Cycling Reaction<br>C.J. Robinson, M.S. Dunstan, N. Swainston, J. Titchmarsh, E. Takano, N.S. Scrutton and A.J. Jervis<br>14. Commodity Chemicals From Engineered Multimodular Type I Polyketide Synthases<br>Satoshi Yuzawa, Amin Zargar, Bo Pang, Leonard Katz and Jay D. Keasling<br>15. Modular Pathway Rewiring of Yeast for Amino Acid Production<br>Quanli Liu, Tao Yu, Kate Campbell, Jens Nielsen and Yun Chen</p>