Marine Enzymes and Specialized Metabolism - Part A

Specificaties
Gebonden, blz. | Engels
Elsevier Science | e druk, 2018
ISBN13: 9780128139592
Rubricering
Elsevier Science e druk, 2018 9780128139592
Onderdeel van serie Methods in Enzymology
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Samenvatting

Marine enzymes and specialized metabolism - Part A, Volume 604 highlights experimental methods on diverse marine enzymes involved in the construction of bioactive natural product molecules. These detailed protocols are written by experts who actively study and apply marine enzymes in biosynthesis and biotechnology. Comprehensive chapters in this latest release cover Chemoenzymatic synthesis of starting materials and characterization of halogenases requiring acyl carrier protein tethered substrates, Assaying biradical aryl coupling activity of CYP450 enzymes, the Characterization and application of marine microbial omega-3 polyunsaturated fatty acid synthesis, Catalase-related allene oxide synthase: on a biosynthetic route to fatty acid cyclopentenones, Haloalkane dehalogenases from marine microorganisms, and more.

Specificaties

ISBN13:9780128139592
Taal:Engels
Bindwijze:Gebonden

Inhoudsopgave

<p>Section I: Marine Cyanobacteria, Enzymes and Pathways<br>1. Collection, Culturing, and Genome Analyses of Tropical Marine Filamentous Benthic Cyanobacteria<br>Nathan A. Moss, Tiago Leao, Evgenia Glukhov, Lena Gerwick and William H. Gerwick<br>2. PKS–NRPS Enzymology and Structural Biology: Considerations in Protein Production<br>Meredith A. Skiba, Finn P. Maloney, Qingyun Dan, Amy E. Fraley, Courtney C. Aldrich, Janet L. Smith and W. Clay Brown<br>3. Terminal Alkyne Biosynthesis in Marine Microbes<br>Xuejun Zhu and Wenjun Zhang<br>4. The Biochemistry and Structural Biology of Cyanobactin Biosynthetic Enzymes<br>Wenjia Gu, Shi-Hui Dong, Snigdha Sarkar, Satish K. Nair and Eric W. Schmidt<br>5. The Enzymology of Prochlorosin Biosynthesis<br>Silvia C. Bobeica and Wilfred A. van der Donk</p> <p>Section II: Biosynthesis by Bacterial Symbionts of Marine Invertebrate Animals<br>6. Chemoenzymatic Dissection of Polyketide β-Branching in the Bryostatin Pathway<br>Samuel T. Slocum, Andrew N. Lowell, Ashootosh N. Tripathi, Vikram V. Shende, Janet L. Smith and David H. Sherman<br>7. Radical S-Adenosylmethionine Peptide Epimerases: Detection of Activity and Characterization of d-Amino Acid Products<br>Brandon I. Morinaka, Anna L. Vagstad and Jörn Piel<br>8. Cobalamin-Dependent C-Methyltransferases From Marine Microbes: Accessibility via Rhizobia Expression<br>Michael F. Freeman<br>9. Biosynthetic Insights of Calyculin- and Misakinolide-Type Compounds in "Candidatus Entotheonella sp."<br>Agustinus R. Uria, Jörn Piel and Toshiyuki Wakimoto</p> <p>Section III: Natural Product Biosynthetic Enzymology of Marine Bacteria and Fungi<br>10. Chemoenzymatic Synthesis of Starting Materials and Characterization of Halogenases Requiring Acyl Carrier Protein-Tethered Substrates<br>Hem R. Thapa, Andrew J. Lail, Neha Garg and Vinayak Agarwal<br>11. Preparation, Assay, and Application of Chlorinase SalL for the Chemoenzymatic Synthesis of S-Adenosyl-L-Methionine and Analogs<br>Tony D. Davis, Sylvia Kunakom, Michael D. Burkart and Alessandra S. Eustaquio<br>12. In Vitro Analysis of Cyanobacterial Nonheme Iron-Dependent Aliphatic Halogenases WelO5 and AmbO5<br>Xinyu Liu<br>13. Characterization and Biochemical Assays of Streptomyces Vanadium-Dependent Chloroperoxidases<br>Shaun M.K. McKinnie, Zachary D. Miles and Bradley S. Moore<br>14. Unusual "Head-to-Torso" Coupling of Terpene Precursors as a New Strategy for the Structural Diversification of Natural Products<br>Robin Teufel<br>15. Identification of Enzymes Involved in Sesterterpene Biosynthesis in Marine Fungi<br>Jingjing Yan, Jingjing Guo, Wei Yuan, Wanying Mai and Kui Hong<br>16. Assaying Oxidative Coupling Activity of CYP450 Enzymes<br>Vinayak Agarwal<br>17. Preparation and Characterization of the Favorskiiase Flavoprotein EncM and Its Distinctive Flavin-N5-Oxide Cofactor<br>Robin Teufel</p>
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        Marine Enzymes and Specialized Metabolism - Part A