G Protein-Coupled Receptors Part A

<p>1. Generating recombinant peptide ligands for GPCRs and their single step purification<br>2. Ca2+ release assay to study downstream pathways of GPCR signal transduction<br>3. Expression and purification of GPCR interacting protein, arrestin<br>4. Isolation and characterization of chemosensory GPCRs from exosomes<br>5. Characterization of chemosensory bitter taste receptor signalling<br>6. Reporter gene assays for investigating GPCR signaling<br>7. Measuring binding affinity of regulatory motifs (peptides) to purified G proteins<br>8. Measuring nucleotide exchange on purified G proteins<br>9. Qualitative and quantitative measurement of GPCR-G protein activity in living cells<br>10. Computational approaches to study GPCR activation<br>11. Visualization and quantification of GPCR trafficking in mammalian cells by confocal microscopy<br>12. Computational studies of βarrestin activation<br>13. Measuring G protein coupling using Glosensor assay<br>14. Computational investigations into GPCR dimerization<br>15. cAMP Assays in GPCR Drug Discovery<br>16. Assays of adrenal GPCR signaling and regulation: Measuring adrenal beta-arrestin activity in vivo through plasma membrane recruitment</p>