Enzymology at the Membrane Interface: Intramembrane Proteases

Specificaties
Gebonden, blz. | Engels
Elsevier Science | e druk, 2017
ISBN13: 9780128122136
Rubricering
Elsevier Science e druk, 2017 9780128122136
Onderdeel van serie Methods in Enzymology
€ 196,00
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Samenvatting

Enzymology at the Membrane Interface: Intramembrane Proteases, Volume 584, the latest release in the Methods in Enzymology series, covers a subset of enzymes that work in the environment of the biological cell membrane. This field, called interfacial enzymology, involves a special series of experimental approaches for the isolation and study of these enzymes.

Specificaties

ISBN13:9780128122136
Taal:Engels
Bindwijze:Gebonden

Inhoudsopgave

<p>1. Biochemical Characterization of Function and Structure of RseP, an Escherichia Coli S2P Protease<br>Y. Hizukuri, K. Akiyama and Y. Akiyama<br>2. Signal Peptidase Enzymology and Substrate Specificity Profiling<br>R.E. Dalbey, D. Pei and Ö.D. Ekici<br>3. Screening and Characterization Strategies for Nanobodies Targeting Membrane Proteins<br>S. Veugelen, M. Dewilde, B.D. Strooper and L. Chávez-Gutiérrez<br>4. Probing the Activity of Eukaryotic Rhomboid Proteases in Vitro<br>B. Cordier and M.K. Lemberg<br>5. Expression, Purification, and Enzymatic Characterization of Intramembrane Proteases<br>R. Zhou, Y. Shi and G. Yang<br>6. Analyzing Amyloid-β Peptide Modulation Profiles and Binding Sites of γ-Secretase Modulators<br>J. Trambauer, A. Fukumori, B. Kretner and H. Steiner<br>7. Probing the Structure and Function Relationships of Presenilin by Substituted-Cysteine Accessibility Method<br>T. Tomita<br>8. A New Method to Determine the Transmembrane Conformation of Substrates in Intramembrane Proteolysis by Deep-UV Resonance Raman Spectroscopy<br>J.W. Cooley, A. Abdine, M. Brown, J. Chavez, B. Lada, R.D. JiJi and I. Ubarretxena-Belandia<br>9. An Inducible Reconstitution System for the Real-Time Kinetic Analysis of Protease Activity and Inhibition Inside the Membrane<br>R.P. Baker and S. Urban<br>10. Production of Recombinant Rhomboid Proteases<br>E. Arutyunova, R. Panigrahi, K. Strisovsky and M.J. Lemieux<br>11. Mechanism and Inhibition of Rhomboid Proteases<br>K. Strisovsky<br>12. Enzymatic Assays for Studying Intramembrane Proteolysis<br>D.M. Bolduc, D.J. Selkoe and M.S. Wolfe<br>13. Methods for S¬tructural and Functional Analyses of Intramembrane Prenyltransferases in the UbiA Super Family<br>Y. Yang, N. Ke, S. Liu and W. Li<br>14. Functional Study of the Vitamin K Cycle Enzymes in Live Cells<br>J.-K. Tie and D.W. Stafford<br>15. Activity Assays for Rhomboid Proteases<br>E. Arutyunova, K. Strisovsky and J. Lemieux</p>
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        Enzymology at the Membrane Interface: Intramembrane Proteases