<p>1. Direct Electron Detectors - Methods in Enzymology (2016)<br>Greg McMullan, Abdul R. Faruqi and Richard Henderson<br>2. Specimen Behavior in the Electron Beam<br>Robert M. Glaeser<br>3. Specimen Preparation for High-Resolution Cryo-EM<br>Christopher J. Russo and Lori A. Passmore<br>4. Strategies for Automated Cryoem Data Collection Using Direct Detectors <br>Anchi Cheng, Yong Zi Tan, Venkat Dandey, Clinton S. Potter and Bridget Carragher<br>5. Processing of Cryo-EM Movie Data<br>Zev A. Ripstein and John L. Rubinstein<br>6. Processing of Structurally Heterogeneous Cryo-EM Data in RELION<br>Sjors H.W. Scheres<br>7. Single Particle Refinement and Variability Analysis in EMAN2.1<br>Steven J. Ludtke<br>8. Frealign: An Exploratory Tool for Single Particle Cryo-EM<br>Nikolaus Grigorieff<br>9. Testing the Validity of Single Particle Maps at Low and High Resolution<br>Peter B. Rosenthal<br>10. Tools for Model Building and Optimization into Near Atomic Resolution Electron Cryo-Microscopy Density Maps<br>Frank DiMaio and Wah Chiu<br>11. Refinement of Atomic Structures Against CryoEM Maps<br>Garib N. Murshudov<br>12. Cryo-EM Structure Determination Using Segmented Helical Image Reconstruction<br>Simon A. Fromm and Carsten Sachse<br>13. Cryo-Electron Tomography and Subtomogram Averaging<br>William Wan and John A.G. Briggs</p>