Enzymes of Epigenetics

<p>Chromatin Structure and Histones<br>1. In Vitro Chromatin Assembly - Strategies and Quality Control<br>Uma Muthurajan, Francesca Mattiroli, Serge Bergeron, Keda Zhou, Yajie Gu, Srinivas Chakravarthy, Pamela Dyer, Thomas Irving and Karolin Luger<br>2. Preparation, Crystallization and Structure Determination of Chromatin Enzyme/Nucleosome Complexes<br>Robert K. McGinty, Ravindra D. Makde and Song Tan<br>3. Preparation of Recombinant Centromeric Nucleosomes and Formation of Complexes with Non-Histone Centromere Proteins<br>Nikolina Sekulic and Ben E. Black<br>4. Functional  Characterization  of  Histone Chaperones using SNAP-Tag-Based Imaging to Assess De Novo Histone Deposition<br>Camille Clément, Isabelle Vassias, Dominique Ray-Gallet  and Geneviève Almouzni<br>5. Analysis of Nucleosome Sliding by ATP-Dependent Chromatin Remodelling Enzymes<br>Coral Y. Zhou and Geeta J. Narlikar</p> <p>Posttranslational Histone Modification Enzymes and Complexes<br>6. In Vitro Activity Assays for Myst Histone Acetyltransferases and Adaptation for High-Throughput Inhibitor Screening<br>Cheryl E. McCullough and Ronen Marmorstein<br>7. Preparation and Biochemical Analysis of Classical Histone Deacetylases<br>Alejandro Villagra, Eva Sahakian and Edward Seto<br>8. Recombinant Preparation, Biochemical Analysis, and Structure Determination of Sirtuin Family Histone/Protein Deacylases<br>Benjamin Suenkel and Clemens Steegborn<br>9. Preparation, Biochemical Analysis and Structure Determination of SET Domain Histone Methyltransferases<br>Elisa Bergamin and Jean Francois Couture<br>10. Expression, Purification and Biochemical Analysis of the LSD1/KDM1A Histone Demethylase<br>Benoit Laurent and Yang Shi<br>11. LSD1 Histone Demethylase Assays and Inhibition<br>Dawn Hayward and Philip A. Cole<br>12. Purification, Biochemical Analysis, and Structure Determination of JmjC Lysine Demethylases<br>Swathi Krishnan and Raymond C. Trievel<br>13. Preparation and Analysis of Native Chromatin Modifying Complexes<br>Yannick Doyon and Jacques Côté</p> <p>Histone Modification Binders (Readers)<br>14. Preparation, Biochemical Analysis and Structure Determination of the Bromodomain, an Acetyl-lysine Binding Domain<br>Chunyan Ren, Lei Zeng and Ming-Ming Zhou<br>15. Preparation, Biochemical Analysis and Structure Determination of Methyllysine Readers<br>Catherine A. Musselman and Tatiana G. Kutateladze</p> <p>DNA Modifications and Nucleic Acid Regulators<br>16. Quantification of Oxidized 5-Methylcytosine Bases and TET Enzyme Activity<br>Monica Yun Liu, Jamie E. DeNizio and Rahul M. Kohli<br>17. Characterization of how DNA Modifications Affect DNA Binding by C2H2 Zinc Finger Proteins <br>Anamika Patel, Hideharu Hashimoto, Xing Zhang and Xiaodong Cheng<br>18. Crystallographic Studies of Telomerase <br>Hunter Hoffman and Emmanuel Skordalakes<br>19. Detection and Analysis of Long Non-Coding RNAs<br>Fan Lai, Ezra Blumenthal and Ramin Shiekhattar<br>20. Identifying Centromeric RNAs Involved in Histone Dynamics in Vivo<br>Delphine Quénet, David Sturgill and Yamini Dalal</p>