Part I: Identification and Biochemical Analysis of Lamin-Associated Proteins <br>1. BioID Identification of Lamin-Associated Proteins<br>Aaron A. Mehus, Ruthellen H. Anderson and Kyle J. Roux<br>2. Proximity-Dependent Biotin Identification (BioID) in Dictyostelium Amoebae<br>Petros Batsios, Irene Meyer and Ralph Gräf<br>3. Purification and Structural Analysis of LEM-Domain Proteins<br>Isaline Herrada, Benjamin Bourgeois, Camille Samson, Brigitte Buendia, Howard J Worman and Sophie Zinn-Justin<br>4. Purification and Structural Analysis of SUN and KASH Domain Proteins<br>F. Esra Demircioglu, Victor E. Cruz and Thomas U. Schwartz<br>5. Purification of Lamins and Soluble Fragments of NETs<br>Alexandr A. Makarov, Andrea Rizzotto, Peter Meinke and Eric C. Schirmer<br>6. Simple Separation of Functionally-Distinct Populations of Lamin-Binding Proteins<br>Jason M. Berk and Katherine L. Wilson<br>Part II: General Methods for Analysis of Intermediate Filament-Associated Proteins <br>7. Development of a Novel Green Fluorescent Protein-Based Binding Assay to Study the Association of Plakins with Intermediate Filament Proteins<br>Bertrand Favre, Nadja Begré, Jamal-Eddine Bouameur, and Luca Borradori<br>8. Functional Analysis of Keratin-Associated Proteins in Intestinal Epithelia: Heat- Shock Protein Chaperoning and Kinase Rescue<br>Anastasia Mashukova, Radia Forteza and Pedro J. Salas<br>9. Purification of Protein Chaperones and their Functional Assays with Intermediate Filaments<br>Ming Der Perng, Yu-Shan Huang and Roy A. Quinlan<br>10. Purification and Structural Analysis of Plectin and BPAG1e<br>José A Manso, Inés García Rubio, María Gómez-Hernández, Esther Ortega, Rubén M Buey, Ana M Carballido, Arturo Carabias, Noelia Alonso-García and José M de Pereda <br>11. Purification and Structural Analysis of Desmoplakin<br>Hee-Jung Choi and William I. Weis<br>12. Degradation of the Intermediate Filament Family by Gigaxonin<br>Pascale Bomont<br>Part III: Functional and Genetic Analysis of the Plakin and Other Cytoskeletal Cross-Linkers <br>13. Functional and Genetic Analysis of Plectin in Skin And Muscle<br>Günther A. Rezniczek, Lilli Winter, Gernot Walko, and Gerhard Wiche<br>14. Functional and Genetic Analysis of Epiplakin in Epithelial Cells<br>Sandra Szabo, Karl L. Wögenstein and Peter Fuchs<br>15. In Vitro Model of the Epidermis: Connecting Protein Function to 3D Structure<br>Christopher Arnette,Jennifer L. Koetsier, Paul Hoover, Spiro Getsios and Kathleen J. Green<br>16. Functional Analysis of Periplakin and Envoplakin, Cytoskeletal Linkers and Cornified Envelope Precursor Proteins<br>Veronika Boczonadi and Arto Määttä<br>17. Microtubule-Actin Crosslinking Factor 1: Domains, Interaction Partners and Tissue-Specific Functions<br>Dmitry Goryunov and Ronald K. H. Liem<br>18. Functional and Genetic Analysis of Neuronal Isoforms of BPAG1<br> Anisha Lynch-Godrei and Rashmi Kothary<br>19. "Functional and Genetic Analysis of Spectraplakins in Drosophila<br>Ines Hahn, Matthew Ronshaugen, Natalia Sánchez-Soriano and Andreas Prokop<br>20. Functional and Genetic Analysis of VAB-10 Spectraplakin in C. Elegans<br>Christelle Gally, Huimin Zhang and Michel Labouesse<br>Part IV: Functional and Genetic Analysis of Lamin-associated proteins<br>21. Tagged Chromosomal Insertion Site (TCIS) System, a Method to Study Lamina Associated Chromatin<br>Jennifer C. Harr and Karen L. Reddy<br>22. Lamin Binding Proteins in Caenorhabditis Elegans<br>Agnieszka Dobrzynska, Peter Askjaer and Yosef Gruenbaum<br>23. Pathologically-Relevant Prelamin A Interactions with Transcription Factors<br>Arantza Infante and Clara I. Rodríguez<br>24. MCLIP Detection of Novel Protein-Protein Interactions at the Nuclear Envelope<br>Mohammed Hakim Jafferali, Ricardo A. Figueroa and Einar Hallberg<br>25. Preparation of the Human Cytomegalovirus Nuclear Egress Complex and Associated Proteins<br>Mayuri Sharma, Jeremy P. Kamil and Donald M. Coen