Paul T. Matsudaira
Elsevier Science
e druk, 1993
9780124802827
A Practical Guide to Protein and Peptide Purification for Microsequencing
Specificaties
Paperback, blz.
|
Engels
Elsevier Science |
e druk, 1993
ISBN13: 9780124802827
Rubricering
Verwachte levertijd ongeveer 9 werkdagen
Samenvatting
Why a Second Edition?
The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry.
Specificaties
Inhoudsopgave
Strategies for Obtaining Partial Amino Acid Sequence Data from Small Quantities (5nmol) of Pure of Partially Purified Protein. Enzymatic Digestion of Proteins and HPLC Peptide Isolation<br>Purification of Proteins and Peptides By SDS-PAGE<br>Internal Amino Acid Sequence Analysis of Proteins After in Situ Protease Digestion on Nitrocellulose<br>Mass Spectrometric Strategies for the Structural Characterization of Proteins