Laboratory Methods in Enzymology: Protein Part A

Practical Steady-state Enzyme Kinetics<br>Quantification of Protein Concentration using UV absorbance and Coomassie Dyes<br>Preparation of protein samples for mass spectrometry and N-terminal sequencing<br>Lysis of mammalian and Sf9 cells<br>In Vivo [35 S]-Methionine Incorporation<br>Pulse-chase analysis to measure protein degradation<br>Labeling of a Protein with Fluorophores using Maleimide Derivitization<br>Labeling a protein with fluorophores using NHS ester derivitization<br>Protein Derivitization-Expressed Protein Ligation<br>Protein Biotinylation<br>Purification of His-tagged proteins<br>Affinity purification of a recombinant protein expressed as a fusion with the maltose-binding protein (MBP) tag<br>Immunoaffinity purification of proteins<br>Affinity Purification of Protein Complexes Using TAP Tags<br>Strep-tagged protein purification<br>Proteolytic affinity tag cleavage<br>Affinity Pull-down of Proteins using anti-FLAG M2 Agarose Beads<br>Protein Affinity Purification using Intein/Chitin Binding Protein Tags<br>Purification of GST-tagged proteins