Methods of Adipose Tissue Biology Part A

1. Adipocyte-Specific Transgenic and Knockout Models<br>2. Imaging White Adipose Tissue With Confocal Microscopy<br>3. Isolation and Study of Adipocyte Precursors<br>4. Imaging of Adipose Tissue<br>5. Adipose Tissue Angiogenesis Assay<br>6. Quantifying Size and Number of Adipocytes in Adipose Tissue<br>7. Use of Osmium Tetroxide Staining with Micro-Computerized Tomography to Visualize and Quantify Bone Marrow Adipose Tissue in Vivo<br>8. Brown Adipose Tissue in Humans: Detection and Functional Analysis Using PET (Positron Emission Tomography), MRI (Magnetic Resonance Imaging), and DECT (Dual Energy Computed Tomography)<br>9. Analysing the Functions and Structure of the Human Lipodystrophy Protein Seipin<br>10. Differentiation of Human Pluripotent Stem Cells into Highly Functional Classical Brown Adipocytes<br>11. Analysis and Measurement of the Sympathetic and Sensory Innervation of White and Brown Adipose Tissue<br>12. Measurement and manipulation of Human Adipose Tissue Blood Flow Using Xenon Washout Technique and Adipose Tissue Microinfusion<br>13. Isolation and Quantitation of Adiponectin Higher Order Complexes<br>14. Genome-Wide Profiling of Transcription Factor Binding and Epigenetic Marks in Adipocytes by ChIP-seq<br>15. Analysis and Isolation of Adipocytes by Flow Cytometry<br>16. Flow Cytometry Analyses of Adipose Tissue Macrophages