1. Isolation of Antarctic fish tubulin and MAPs<br>2. Making, isolating and characterizing tubulin alpha antibodies<br>3. Techniques for labeling tubulin and drugs<br>4. Single-particle 3D reconstruction methods for decorated and undecorated microtubules<br>5. A new microtubule gliding assay and analysis of microtubule persistence length<br>6. Characterization of gamma tubulin <br>7. Biochemical and Structural Studies of the doublecortin family of MAPs<br>8. Detection and quantification of microtubule detachment from centrosomes and spindle poles<br>9. Regulation of tubulin expression by micro RNAs: Implications for Drug Resistance<br>10. A portable magnetic tweezers device to visualize three-dimensional deformation of reconstituted microtubule networks<br>11. Mitochondrial/microtubule interactions – mmbp1<br>12. Going Solo: Measuring the motions of microtubules with an in vitro assay for TIRF microscopy<br>13. Analysis of Microtubules and Motors in Isolated Axoplasm from the Squid Giant Axon<br>14. Analysis of drug binding to microtubules and steady state microtubule growth/treadmilling by use of a sedimentation assay in vitro<br>15. Specific recombinant antibody technology for the study of microtubule dynamics and regulatory proteins<br>16. Tubulin-specific Chaperones: Components of a Molecular Machine that Assembles the aB Heterodimer<br>17. G-protein tubulin interactions<br>18. Katanin, a microtubule severing enzyme: expression, purification, and biophysical characterization<br>19. Measuring tubulin polymerization in vitro – comparison of methods and possible artifacts<br>20. Imaging microtubules in living plant cells<br>21. How to use which antibody known in the field, what to expect from this antibody and how semi-quantitative data can be reliably obtained with antibody staining<br>22. Preparation of bacterial tubulin BtubA/B and constructs bearing eukaryotic tubulin sequences<br>23. Microtubule-associated proteins and tubulin interaction by isothermal titration calorimetry<br>24. TBC<br>25. Characterization of the mechanism of action of the covalent-binding microtubule-stabilizing agent zampanolide<br>26. Studying kinetochore-fibre ultrastructure using correlative light-electron microscopy<br>27. Fluorescence-based Assays for Microtubule Structure<br>28. Yeast Tubulin-structure-function analysis<br>29. Microtubule Binding SIm to simulate binding of proteins to polymers under different binding models
