Single Molecule Tools, Part B: Super-Resolution, Particle Tracking, Multiparameter, and Force Based Methods

Specificaties
Gebonden, blz. | Engels
Elsevier Science | e druk, 2010
ISBN13: 9780123814821
Rubricering
Elsevier Science e druk, 2010 9780123814821
Onderdeel van serie Methods in Enzymology
€ 207,25
Levertijd ongeveer 8 werkdagen

Samenvatting

Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme assays, image components of spatially distributed samples, probe the mechanical properties of single molecules in their native environment, and "just look at the thing" as anticipated by the visionary Richard Feynman already half a century ago. Single Molecule Tools, Part B: Super-Resolution, Particle Tracking, Multiparameter, and Force Based Methods captures a snapshot of this vibrant, rapidly expanding field, presenting articles from pioneers in the field intended to guide both the newcomer and the expert through the intricacies of getting single molecule tools.

Specificaties

ISBN13:9780123814821
Taal:Engels
Bindwijze:Gebonden

Inhoudsopgave

<p>1. Watching single DNA replication loops under flow extension</p> <p>Antoine Van Oijen</p> <p>2. Star polymer surface passivation for single molecule detection</p> <p>Jürgen Groll , Martin Möller</p> <p>3. Ultrahigh resolution detection of single active motor proteins in live cells</p> <p>Paul Selvin, Erdal Toprak & Comert Kural</p> <p>4. Molecules and Methods for Superresolution Imaging</p> <p>W.E. Moerner, Michael Thompson, Sam Lord, Julie Biteen, and Nick Conley +…</p> <p>5. Aqueous nanodroplets for studying single molecules</p> <p>Lori Goldner</p> <p>6. High-speed atomic force microscopy techniques for visualizing dynamic behavior of biological macromolecules </p> <p>Toshio Ando</p> <p>7. Single-Biomolecule Spectroscopy Using Microfluidic Platforms</p> <p>Richard Zare, Dr. Olga Dudko, Dr. Jérôme Mathé</p> <p>8. DNA Looping Kinetics Analyzed by Tethered Particle Microscopy</p> <p>Laura Finzi</p> <p>9. Single molecule observation of proteins in vivo</p> <p>Shimon Weiss, Gopal Iyer</p> <p>10. DNA curtains as a high-throughput approach to single molecule imaging</p> <p>Eric Greene</p> <p>11. Single-molecule enzymology of protein synthesis</p> <p>Ruben Gonzalez</p> <p>12. Single molecule fluorescence studies of intrinsically disordered proteins</p> <p>Ashok Deniz</p> <p>13. Nanovesicle trapping for studying transient protein-protein interactions by single molecule FRET</p> <p>Peng Chen</p> <p>14. Tracking single motor proteins in the cytoplasm of mammalian cells</p> <p>Edgar Meyhöfer</p> <p>15. Conformational States of F1-ATPase by Single-Molecule Rotation</p> <p>Hiroyuki Noji</p> <p>16. Single Molecule Sequencing by Fluorescence Imaging</p> <p>Patrice Milos</p> <p>17. Real-Time DNA Sequencing from Single Polymerase Molecules</p> <p>Jonas Korlach</p> <p>18. Micropatterning and single molecule imaging for quantitative analysis of protein-protein interactions in living cells</p> <p>Gerhard Schütz</p> <p>19. Probing virus-receptor interactions by atomic force spectroscopy</p> <p>Peter Hinterdorfer</p> <p>20. Single-Molecule Fluorescence Spectroscopy of Cytochrome P450 in Nanodiscs</p> <p>Elizabeth Rhoades, William M. Atkins</p> <p>21. Analysis of complex single molecule FRET time traces</p> <p>Nils Walter, Mario Blanco</p> <p>22. Application of super-resolution imaging to single particle tracking in nanotechnology</p> <p>Nils Walter, Chamaree de Silva & Anthony Manzo</p> <p>23. Scanning FCS for the characterization of protein dynamics in live cells</p> <p>Petra Schwille</p> <p>24. Single mRNA molecule tracking in live cells</p> <p>Rob Singer</p> <p>25. Single-molecule high-resolution colocalization (SHREC) or Single-molecule optical-trap analyses of protein structure</p> <p>James Spudich</p> <p>26. Nanopore force Spectroscopy tools for analyzing single bio-complexes</p> <p>Amit Meller</p> <p>27. Use of plasmon coupling to reveal DNA dynamics at the single molecule level</p> <p>Jan Liphardt</p> <p>28. "Fluorescence-force spectroscopy" or "Suppression of fluorophore blinking and bleaching" or "Spontaneous intersubunit rotation of the ribosome"</p> <p>Taekjip Ha</p> <p>29. Multiplexed single mRNA imaging in fixed cells</p> <p>Sanjay Tyagi, Arjun Raj</p> <p>30. Size-Minimized Quantum Dots for Single-Molecule and Intracellular Imaging</p> <p>Shuming Nie</p> <p>31. The ABEL trap</p> <p>Adam Cohen, Alex Fields</p> <p>32. An optical torque wrench</p> <p>Steve Block, Arthur La Porta</p> <p>33. Determining the Stoichiometry of Protein Hetero-complexes in Living Cells with Fluorescence Fluctuation Spectroscopy</p> <p>Joachim Mueller</p> <p>34. Fluorescent Visualization of Single Protein-DNA Complexes</p> <p>Steve Kowalzcykowski</p> <p>35. Direct Measurement of Tertiary Contact Cooperativity in RNA Folding by single molecule FRET</p> <p>Daniel Herschlag</p> <p>36. Nanometer-localized multiple single-molecule (NALMS) or Single-molecule nonequilibrium periodic Mg2+-concentration jump experiments</p> <p>Norbert Scherer</p> <p>37. Multiparameter single molecule fluorescence detection with applications to FRET</p> <p>Claus Seidel</p> <p>38. single-particle tracking-photoactivated localization microscopy (sptPALM) within live cells</p> <p>Jennifer Lippincott-Schwartz, Suliana Manley</p> <p>39. Site-specific incoporation of fluorescent probes into RNA polymerase</p> <p>Richard Ebright</p> <p>40. Quantitative single-molecule imaging by confocal laser scanning microscopy</p> <p>Rudolph Rigler</p> <p>41. Studies of DNA-replication at the single molecule level using magnetic tweezers</p> <p>David Bensimon, Vincent Croquette</p> <p>42. RNA labeled for single molecule FRET analysis from ligation with T4 RNA ligases</p> <p>John Abelson</p> <p>43. Combining optical tweezers, single-molecule fluorescence microscopy and microfluidics for studying reversible protein-DNA interactions</p> <p>Gijs Wuite, G. Farge, P. Gross & E.J.G. Peterman</p> <p>44. How dwell time distributions and other such observables in single molecule analysis can be used to extract information from molecular systems</p> <p>Carlos Bustamante, Jeff Moffitt & Yann Robert Chemla</p> <p>45. Atomic force microscopy studies of human rhinovirus: topology and molecular forces</p> <p>Peter Hinterdorfer</p>
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        Single Molecule Tools, Part B: Super-Resolution, Particle Tracking, Multiparameter, and Force Based Methods