STRUCTURE AND FUNCTION<br>1. A Novel Chemokine-mediated In Vivo T Cell Recruitment Assay for Structure Function Studies<br>(Andrew D. Luster and Gabriele Campanella)<br>2. Tyrosine sulfation of HIV-1 coreceptors and other chemokine-binding receptors (Micheal R. Farzan)<br>3. Lymphotactin Structural Dynamics (Brian F. Volkman)<br>4. Regulation of chemokines by posttranslational modifications (Paul Proost )<br>5. Proteolytic Processing of Chemokines: Mass Spectrometry, Biochemistry, Neoepitope Antibody and Functional Characterization (Christopher Overall and Amanda Starr)<br>6. Multiple approaches to study Chemokine Receptor Homo and Heterodimerization (Mario Mellado)<br>7. Heterodimerization of Chemokine Receptors with Other GPCRs (Graeme Milligan and Shirley Appelbe)<br>8. Homo and Hetero-Oligomerization of Chemokines (Tracy Handel, Damon Hamel, Ariane Jansma)<br>9. Interactions of Chemokine with Glycosaminoglycans (Amanda Proudfoot)<br>10. Interactions of Chemokine with Glycosaminoglycans (Andreas J. Kungl)<br>11. Structures of Chemokine:Chemokine Binding Protein Complexes (Daved Fremont)<br>12. Activation Mechanisms of Chemokine Receptors (Mette Rosenkilde)<br>13. Modeling Small Molecule Compound Binding to Receptors (Richard Horuk, James Pease, N. Vaidehi)<br>14. Spectroscopic Approaches to Study Conformational Changes in GPCRs (David Farrens)<br>15. Plasmon Resonance Methods in GPCR Signaling (Victor Hruby, Isabel Alves, Gordon Tollin)<br>16. Flow Cytometric Analyses of GPCR Interactions or Rapid-mix flow cytometry measurements of subsecond regulation of G protein-coupled receptor ternary complex dynamics (Larry Sklar)<br>17. Expression and Functional Reconstitution of CCR1 (Tracy Handel and Samantha Allen, Damon Hamel)<br>18. Characterization of the Interactions of Chemokines with Glycosaminoglycans by Fourier Transform Ion Cyclotron Resonance (Julie A. Leary)<br>CHEMOKINE FUNCTION IN CELL MIGRATION<br>19. In vitro assays for studying chemokine-triggered lymphocyte transendothelial migration under physiological flow conditions (Ronen Alon and Francis Lin)<br>20. Microfluidic device (Eugene Butcher)<br>21. Two-photon imaging and quantitative analysis of leukocyte migration in vivo (Mark Miller and Jenna Cash)<br>22. Zymosan induced peritonitis as a model for studying chemokines and inflammatory mediators in vivo (David Greaves)