Grant Jensen
Elsevier Science
e druk, 2010
9780123749062
Cryo-EM Part A: Sample Preparation and Data Collection
Specificaties
Gebonden, blz.
|
Engels
Elsevier Science |
e druk, 2010
ISBN13: 9780123749062
Rubricering
Onderdeel van serie
Methods in Enzymology
Levertijd ongeveer 8 werkdagen
Samenvatting
Cryo-EM Part A: Sample Preparation and Data Collection is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied.
Specificaties
ISBN13:9780123749062
Taal:Engels
Bindwijze:Gebonden
Uitgever:Elsevier Science
Serie:Methods in Enzymology
Inhoudsopgave
<p>Historical perspective</p> <p>3D reconstruction from electron micrographs: a personal account of its development</p> <p>David DeRosier </p> <ol> <p> <li>Preparation of 2-D crystals</li> <p>Henning Stahlberg</p> <p> <li>Helical Crystallization of Soluble and Membrane Binding Proteins</li> <p>Elizabeth M. Wilson-Kubalek, Christopher Arthur, Joshua S. Chappie</p> <p> <li>Plunge Freezing for Electron Cryomicroscopy</li> <p>Alasdair W. McDowall, Megan J. Dobro, Linda A. Melanson, Grant J. Jensen</p> <p> <li>A Practical Guide to the Use of Monolayer Purification and Affinity Grids</li> <p>Thomas Walz, Deborah F. Kelly, Danijela Dukovski</p> <p> <li>GraFix: Stabilization of fragile macromolecular complexes for single particle cryo-EM</li> <p>Holger Stark</p> <p> <li>Cryo-negative staining of macromolecular assemblies</li> <p>Sacha De Carlo, Holger Stark</p> <p> <li>Liposomes on a streptavidin crystal: a system to study membrane proteins by cryo-EM</li> <p>Fred J. Sigworth, Liguo Wang</p> <p> <li>Micromanipulator-Assisted Vitreous Cryosectioning and Sample Preparation by High-Pressure Freezing</li> <p>Mark S. Ladinsky</p> <p> <li>Site-Specific Biomolecule Labeling with Gold Clusters</li> <p>Christopher J. Ackerson, Richard D. Powell, James F. Hainfeld</p> <p> <li>How to Operate a Cryo-Electron Microscope</li> <p>Huilin Li, Jingchuan Sun</p> <p> <li>Collecting electron crystallographic data of two-dimensional protein crystals</li> <p>Thomas Walz, Richard K. Hite, Andreas D. Schenk, Zongli Li, Yifan Cheng</p> <p> <li>Automated Data Collection for Electron Microscopic Tomography</li> <p>David Agard, Shawn Q. Zheng, J. W. Sedat</p> <p> <li>Correlated Light and Electron Cryomicroscopy</li> <p>Grant J. Jensen, Ariane Briegel, Songye Chen, Abraham J. Kosterc, Jürgen M. Plitzkod, Cindi L. Schwartz</p> <p> <li>Phase Plates for Transmission Electron Microscopy</li> <p>Radostin Danev, Kuniaki Nagayama</p> <p> <li>Radiation damage in electron cryomicroscopy</li> </ol> <p>John L. Rubinstein, Lindsay A. Baker</p>