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Biothermodynamics Part A

Specificaties
Gebonden, blz. | Engels
Elsevier Science | e druk, 2009
ISBN13: 9780123745965
Rubricering
Elsevier Science e druk, 2009 9780123745965
Onderdeel van serie Methods in Enzymology
€ 213,50
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Samenvatting

In the past several years, there has been an explosion in the ability of biologists, molecular biologists and biochemists to collect vast amounts of data on their systems. This volume presents sophisticated methods for estimating the thermodynamic parameters of specific protein-protein, protein-DNA and small molecule interactions. The use of thermodynamics in biological research is used as an “energy book-keeping system.” While the structure and function of a molecule is important, it is equally important to know what drives the energy force. These methods look to answer: What are the sources of energy that drive the function? Which of the pathways are of biological significance? As the base of macromolecular structures continues to expand through powerful techniques of molecular biology, such as X-ray crystal data and spectroscopy methods, the importance of tested and reliable methods for answering these questions will continue to expand as well.

Specificaties

ISBN13:9780123745965
Taal:Engels
Bindwijze:Gebonden

Inhoudsopgave

1. Methods for assessing T cell receptor recognition; B. M. Baker <br>2. Energetic approaches to the characterization of the E. coli PriA helicase; W. M. Bujalowski <br>3. Practical approaches to protein folding and assembly: Spectroscopic strategies in thermodynamics and kinetics <br>C. Clark, J. Walters, S. L. Milam <br>4. Using thermodynamics to understand progesterone receptor function: Method and theory; D. Bain, K. Connaghan-Jones, A. Moody <br>5. Measurement of Mg2+ - RNA interaction free energies by use of a fluorescent indicator dye; D. Draper, D. Grilley, A. M. Soto <br>6. Analysis of repeat-protein folding using nearest-neighbor statistical mechanical models; D. Barrick <br>7.Methods for assessing folding and binding energetics in proteins; E. Freire, A. Schon, A. Velazquez-Campoy <br>8. Kinetic equilibrium analysis of the myosin ATPase; E. M. De La Cruz, E. M. Ostap <br>9. Methodological approaches to assessing cooperativity using the Hill Coefficient; J. M. Holt, G. Ackers <br>10. Experimental evaluation of the forces that define membrane protein structure; L. Tamm, J. U. Bowie, H. Hong, N. HyunJoong Joh <br>11. Energetic Approaches to DNA-Drug Binding Interactions; E. Lewis, W. D. Wilson, J. T. Petty <br>12. NMR analysis of dynein light chain phosphorylation and interactions with diverse ligands; E. Barbar, G. Benison <br>13. Cooperative DNA binding of the DNA-repair protein AGT; M. G. Fried <br>14. Measurement of Parvalbumin Divalent Ion Affinity by Isothermal Titration Calorimetry; M. T. Henzl <br>15. Methodological approaches to the study of conformational fluctuations; V. J. Hilser <br>16. Applications of molecular dynamics simulations to study protein quaternary structure changes involved in allostery; J. Carey, R. Ettrich <br>17. Thermodynamics of protein DNA binding with hyperthermophile proteins; J. Shriver, S. Edmondson <br>18. Experimental approaches to the study of protein dynamics; J. Wand <br>19. Methodological approaches to the thermodynamics of cholesterol-lipid interactions; J. Huang, G. W. Geigenson <br>10. Methods applied to the analysis of allostery and cooperativity in calmodulin; M. A. Shea <br>21. Analysis of DNA binding by footprinting methods; M. Brenowitz <br>22. RNA function from thermodynamic cycles: from the conceptual to the practical; P. Bevilacqua, N. Siegfried <br>23. Biophysical techniques applied to virus assembly and stability; A. Zlotnick <br>24. Bioenergetic methods applied to allostery; R. Noble <br>25. The effect of kinetics on sedimentation velocity profiles: the role of intermediates; J. J. Correia, W. Stafford <br>26. Methods for the bioenergetic characterization of bacteriorhodopsin; G. J. Turner <br>27. Ultracentrifugation methods applied to bioenergetics of contractile proteins; W. Stafford
€ 213,50
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        Biothermodynamics Part A