<p>1. Why Purify Enzymes?</p> <p>Section I: Developing Purification Procedures<br>2. Strategies and Considerations for Protein Purifications<br>3. Use of Bioinformatics in Planning a Protein Purification<br>4. Preparing A Purification Summary Table</p> <p>Section II: General Methods for Handling Proteins and Enzymes<br>5. Setting Up A Laboratory<br>6. Buffers: Principle and Practice<br>7. Measurement of Enzyme Activity<br>8. Quantitation of Protein<br>9. Concentration of Proteins and Removal of Solutes<br>10. Maintaining Protein Stability</p> <p>Section III: Recombinant Protein Expression and Purification<br>11. Selecting an Appropriate Method for Expressing a Recombinant Protein<br>12. Bacterial Systems for Production of Heterologous Proteins<br>13. Expression in the Yeast Pichia Pastoris<br>14. Baculovirus-Insect Cell Expression Systems<br>15. Recombinant Protein Production By Transient Gene Transfer Into Mammalian Cells<br>16. Tagging For Protein Expression<br>17. Refolding of Solubilized Inclusion Body Proteins</p> <p>Section IV: Preparation of Extracts and Subcellular Fractionation <br>18. Advances In Preparation of Biological Extracts for Protein Purification<br>19. Isolation of Subcellular Organelles and Structures</p> <p>Section V: Purification Procedures: Bulk Methods<br>20. Protein Precipitation Techniques<br>21. Affi-Gel Blue for Nucleic Acid Removal and Early Enrichment of Nucleotide Binding Protein</p> <p>Section VI: Purification Procedures: Chromatographic Methods<br>22. Ion Exchange Chromatography<br>23. Gel Filtration<br>24. Protein Chromatography on Hydroxyapatite Columns<br>25. Theory and Use of Hydrophobic Interaction Chromatography in Protein Purification Applications</p> <p>Section VII: Purification Procedures: Affininty Methods<br>26. Affinity Chromatography: General Methods<br>27. Immobilized-Metal Affinity Chromatography (IMAC)<br>28. Identification, Production, and Use of Polyol-Responsive Monoclonal Antibodies for Immunoaffinity Chromatography</p> <p>Section VIII: Purification Procedures: Electrophoretic Methods<br>29. One-Dimensional Gel Electrophoresis<br>30. Protein Analysis Using High-Resolution Two-Dimensional Polyacrylamide Gel Electrophoresis and Isoelectric Focusing<br>31. Protein Gel Staining Methods: An Introduction And Overview<br>32. Elution of Proteins From Gels<br>33. Immunodetection By Protein Blotting Performing and Optimizing Western Blots With An Emphasis on Chemiluminescent Detection</p> <p>Section IX: Purification Procedures: Membrane Proteins And Glycoproteins<br>34. Detergents: An Overview<br>35. Purification of Membrane Proteins<br>36. Purification of Recombinant G-Protein-Coupled Receptors<br>37. Cell-Free Translation Of Integral Membrane Proteins Into Unilamelar Liposomes</p> <p>Section X: Characterization of Purified Proteins<br>38. Determination of Protein Purity<br>39. Determination of Size, Molecular Weight, and Presence of Subunits<br>40. Identification and Quantification of Protein Posttranslational Modifications<br>41. Parallel Methods For Expression And Purification<br>42. Techniques To Isolate O2-Sensitive Proteins: [4Fe-4S]-FNR As An Example<br>43. Rethinking Your Purification Procedure<br>44. Important But Little Known (Or Forgotten) Artifacts in Protein Biochemistry</p>
