Translation Initiation: Reconstituted Systems and Biophysical Methods

Chapter 1<br>Transient Kinetics, Fluorescence, and FRET in Studies of Initiation of Translation in Bacteria<br>Pohl Milon, Andrey L. Konevega, Frank Peske, Attilio Fabbretti, Claudio O. Gualerzi, and Marina V. Rodnina<br><br>Chapter 2<br>Binding of mRNA to the Bacterial Translation Initiation Complex<br>Sean M. Studer and Simpson Joseph<br><br>Chapter 3<br>Real time dynamics of ribosome-ligand interaction by time-resolved chemical probing methods<br>Attilio Fabbretti, Pohl Milon, Anna Maria Giuliodori, Claudio O. Gualerzi, Cynthia L. Pon<br><br>Chapter 4<br>Over-Expression and Purification of Mammalian Mitochondrial Translational Initiation Factor 2 and Initiation Factor 3<br>Domenick G. Grasso, Brooke E. Christian, Angela Spencer and Linda L. Spremulli<br><br>Chapter 5<br>In Vitro Studies of Archaeal Translational Initiation<br>Dario Benelli and Paola Londei<br><br>Chapter 6<br>Reconstitution of yeast translation initiation<br>Michael G. Acker, Sarah E. Kolitz, Sarah F. Mitchell, Jagpreet S. Nanda and Jon R. Lorsch<br><br>Chapter 7<br>Assembly and analysis of eukaryotic translation initiation complexes<br>Andrey V. Pisarev, Anett Unbehaun, Christopher U.T. Hellen, and Tatyana V. Pestova<br><br>Chapter 8<br>Reconstitution of Mammalian 48S Ribosomal Translation Initiation Complexes<br>Romit Majumdar, Jayanta Chaudhuri and Umadas Maitra <br>Chapter 9<br>Biophysical approach to studies of cap-eIF4E interaction by synthetic cap analogues<br>Anna Niedzwiecka, Janusz Stepinski, Jan M. Antosiewicz, Edward Darzynkiewicz and Ryszard Stolarski<br><br>Chapter 10<br>Biophysical studies of the translation initiation pathway using immobilised mRNA analogues<br>John E.G. McCarthy, Steven Marsden and Tobias von der Haar<br><br>Chapter 11<br>Protection-Based Assays to Measure Amino Acyl-tRNA<br>Binding to Translation Initiation Factors<br>Yves Mechulam*, Laurent Guillon, Laure Yatime, Sylvain Blanquet and Emmanuelle Schmitt<br><br>Chapter 12<br>NMR methods for studying protein-protein interactions involved in translation initiation<br>Assen Marintchev, Dominique Frueh, and Gerhard Wagnerƒx<br><br>Chapter 13<br>Structural methods to study IRES Function<br>Jeffrey S. Kieft*, David A. Costantino, Megan E. Filbin, John Hammond, and Jennifer S. Pfingsten<br><br>Chapter 14<br>Biophysical and biochemical investigations of dsRNA-activated kinase PKR.<br>Sean A. McKenna, Darrin A. Lindhout, Takashi Shimoike, and Joseph D. Puglisi<br><br>Chapter 15<br>Expression and purification of recombinant wheat translation initiation factors eIF1, eIF1A, eIF4A, eIF4B, eIF4F, eIF(iso)4F and eIF5<br>Laura K. Mayberry, Michael D. Dennis, M. Leah Allen, Kelley Ruud Nitka, Patricia A. Murphy, Lara Campbell and Karen S. Browning<br><br>Chapter 16<br>In vitro reconstitution and biochemical characterization of translation initiation by internal ribosomal entry<br>Victoria G. Kolupaeva, Sylvain de Breyne, Tatyana V. Pestova, Christopher U.T. Hellen