Stem Cell Culture

<p>Contributors to Volume 86</p> <p>Preface</p> <p>Biography</p> <p>Derivation of Human Embryonic Stem Cells in Standard and Chemically Defined Conditions</p> <p>I Introduction</p> <p>II Methods</p> <p>III Discussion</p> <p>IV Materials</p> <p>Autogeneic Feeders for the Culture of Undifferentiated Human Embryonic Stem Cells in Feeder and Feeder‐Free Conditions</p> <p>I Introduction</p> <p>II Methods and Materials</p> <p>III Results</p> <p>IV Discussion and Summary</p> <p>Microporous Membrane Growth Substrates for Embryonic Stem Cell Culture and Differentiation</p> <p>I Introduction</p> <p>II Rationale</p> <p>III Methods</p> <p>IV Summary</p> <p>Culture of Rodent Spermatogonial Stem Cells, Male Germline Stem Cells of the Postnatal Animal</p> <p>I Introduction</p> <p>II Rationale</p> <p>III Methods</p> <p>IV Materials</p> <p>V Discussion</p> <p>Characterization of Human Amniotic Fluid Stem Cells and Their Pluripotential Capability</p> <p>I Introduction</p> <p>II Amniotic Fluid Cell Composition and Stem Cells</p> <p>III In Vitro Potential of Amniotic Fluid Stem Cells</p> <p>IV Ex Vivo and In Vivo Application of Amniotic Fluid Stem Cells</p> <p>V Discussion</p> <p>Method to Isolate Mesenchymal‐Like Cells from Wharton's Jelly of Umbilical Cord</p> <p>I Introduction</p> <p>II Rationale</p> <p>III Methods</p> <p>IV Materials</p> <p>V UCMSCs and Culture Characteristics</p> <p>VI Discussion</p> <p>Isolation, Characterization, and Differentiation of Human Umbilical Cord Perivascular Cells (HUCPVCs)</p> <p>I Introduction</p> <p>II Methods</p> <p>III Concluding Remarks</p> <p>Hepatic Stem Cells and Hepatoblasts: Identification, Isolation, and Ex Vivo Maintenance</p> <p>I Introduction</p> <p>II Epithelial–Mesenchymal Relationship</p> <p>III Stem Cells and Lineage Biology</p> <p>IV Liver Processing</p> <p>V Fractionation of Liver Cell Subpopulations</p> <p>VI Feeder Cells</p> <p>VII Extracellular Matrix</p> <p>VIII Culture Media</p> <p>IX Monolayer Cultures of Cells</p> <p>X Three‐Dimensional Systems</p> <p>Acknowledgments</p> <p>Appendix 1: Nomenclature, Glossary, and Abbreviations</p> <p>Appendix 2: Details on the Preparation of Buffers/Reagents</p> <p>Appendix 3: Commercial Sources of Reagents</p> <p>Appendix 4: Sources of Primary and Secondary Antibodies for Immunoselection and for Characterization of the Cells. (See Below for Suppliers and the Location of the Suppliers)</p> <p>Culture of Pluripotent Neural Epithelial Progenitor Cells from E9 Rat Embryo</p> <p>I Introduction</p> <p>II Rationale</p> <p>III Methods</p> <p>IV Materials</p> <p>V Discussion</p> <p>VI Summary</p> <p>Primary and Multipassage Culture of Human Fetal Kidney Epithelial Progenitor Cells</p> <p>I Introduction</p> <p>II Rationale</p> <p>III Methods</p> <p>IV Materials</p> <p>V Discussion</p> <p>VI Summary</p> <p>Isolation of Human Mesenchymal Stem Cells from Bone and Adipose Tissue</p> <p>I Introduction</p> <p>II Isolation of hMSCs from Trabecular Bone</p> <p>III Isolation of hMSCs from Adipose Tissue</p> <p>IV Growth, Subculture, and Cryopreservation of Undifferentiated hMSCs</p> <p>V Surface Marker Characterization of hMSCs</p> <p>VI hMSC Differentiation Assays for Osteogenic, Adipogenic, and Chondrogenic Pathways</p> <p>VII Summary</p> <p>Culture of Mesenchymal Stem/Progenitor Cells in Adhesion‐Independent Conditions</p> <p>I Introduction</p> <p>II Suspension Culture of Bone Marrow‐Derived MPCs</p> <p>III Analysis of Suspension Culture</p> <p>IV Mechanism of MPC Expansion in Suspension</p> <p>V Discussion</p> <p>Purification and Long‐Term Culture of Multipotent Progenitor Cells Affiliated with the Walls of Human Blood Vessels: Myoendothelial Cells and Pericytes</p> <p>I Introduction</p> <p>II Materials and Methods: Cell Analysis and Purification by Flow Cytometry</p> <p>III Materials and Methods: Long‐Term Culture of Human Blood Vessel‐Associated Progenitor Cells</p> <p>IV Materials and Methods: Proliferation Kinetics of Human Myoendothelial Cells and Pericytes</p> <p>V Materials and Methods: Genotypic and Phenotypic Analyses of Long‐Term Cultured Cells</p> <p>VI Conclusion</p> <p>Isolation and Culture of Colon Cancer Stem Cells</p> <p>I Introduction</p> <p>II Identification of CSCs in Colon Cancer Tissue</p> <p>III Isolation and Propagation of Colon CSCs</p> <p>IV Discussion</p> <p>Isolation and Establishment of Human Tumor Stem Cells</p> <p>I Introduction</p> <p>II Rationale</p> <p>III Methods</p> <p>IV Results</p> <p>V Discussion</p> <p>VI Materials</p> <p>Stem Cells from Cartilaginous and Bony Fish</p> <p>I Introduction to Models and Uses</p> <p>II Embryonal Stem Cells</p> <p>III Tissue‐Specific Stem Cells</p> <p>IV Outlook and Future Contributions</p> <p>Index</p> <p>Volume in Series</p>