Two-Component Signaling Systems, Part B

<p>Section I: Structural Approaches</p> <p>[1]: The PICM Chemical Scanning Method for Identifying Domain–Domain and Protein–Protein Interfaces: Applications to the Core Signaling Complex of E. coli Chemotaxis</p> <p>[2]: Use of Site-Directed Cysteine and Disulfide Chemistry to Probe Protein Structure and Dynamics: Applications to Soluble and Transmembrane Receptors of Bacterial Chemotaxis</p> <p>[3]: Measuring Distances by Pulsed Dipolar ESR Spectroscopy: Spin-Labeled Histidine Kinases</p> <p>[4]: Rigid Body Refinement of Protein Complexes with Long-Range Distance Restraints from Pulsed Dipolar ESR</p> <p>[5]: TonB/TolA Amino-Terminal Domain Modeling</p> <p>[6]: Functional Dynamics of Response Regulators Using NMR Relaxation Techniques</p> <p>[7]: The Design and Development of Tar-EnvZ Chimeric Receptors</p> <p>[8]: Functional and Structural Characterization of EnvZ, an Osmosensing Histidine Kinase of E. coli</p> <p>[9]: Light Modulation of Histidine-Kinase Activity in Bacterial Phytochromes Monitored by Size Exclusion Chromatography, Crosslinking, and Limited Proteolysis</p> <p>[10]: A Temperature-Sensing Histidine Kinase—Function, Genetics, and Membrane Topology</p> <p>[11]: The Regulation of Histidine Sensor Kinase Complexes by Quorum Sensing Signal Molecules</p> <p>Section II: Reconstitution of Heterogeneous Systems</p> <p>[12]: Liposome-Mediated Assembly of Receptor Signaling Complexes</p> <p>[13]: Analyzing Transmembrane Chemoreceptors Using In Vivo Disulfide Formation Between Introduced Cysteines</p> <p>[14]: Using Nanodiscs to Create Water-Soluble Transmembrane Chemoreceptors Inserted in Lipid Bilayers</p> <p>[15]: Assays for CheC, FliY, and CheX as Representatives of Response Regulator Phosphatases</p> <p>[16]: Genetic Dissection of Signaling Through the Rcs Phosphorelay</p> <p>Section III: Intracellular Methods and Assays</p> <p>[17]: In Vivo Measurement by FRET of Pathway Activity in Bacterial Chemotaxis</p> <p>[18]: In Vivo and In Vitro Analysis of the Rhodobacter sphaeroides Chemotaxis Signaling Complexes</p> <p>[19]: In Vivo Crosslinking Methods for Analyzing the Assembly and Architecture of Chemoreceptor Arrays</p> <p>[20]: A “Bucket of Light” for Viewing Bacterial Colonies in Soft Agar</p> <p>[21]: Phenotypic Suppression Methods for Analyzing Intra- and Inter-Molecular Signaling Interactions of Chemoreceptors</p> <p>[22]: Single-Cell Analysis of Gene Expression by Fluorescence Microscopy</p> <p>Section IV: Genome-Wide Analyses of Two-Component Systems</p> <p>[23]: Two-Component Systems of Mycobacterium tuberculosis—Structure-Based Approaches</p> <p>[24]: Transcriptomic Analysis of ArlRS Two-Component Signaling Regulon, a Global Regulator, in Staphylococcus aureus</p> <p>[25]: Global Analysis of Two-Component Gene Regulation in H. pylori by Mutation Analysis and Transcriptional Profiling</p> <p>[26]: Phosphotransfer Profiling: Systematic Mapping of Two-Component Signal Transduction Pathways and Phosphorelays</p> <p>[27]: Identification of Histidine Phosphorylations in Proteins Using Mass Spectrometry and Affinity-Based Techniques</p> <p>Subject Index</p> <p>Author Index</p>